CATÁLOGO DE INVESTIGACIONES | AÑO ACADÉMICO 2022-2023 31 Antifungal Activity of Origanum Spp. Essential Oil and Extract Against Anthracnose on Coffee Plants Anthracnose is a disease severely affecting coffee crop farms in Puerto Rico and other tropical countries. The disease is difficult to control, so some experts choose to start fresh instead of trying to save the contaminated crop. This study aims to test the antifungal activity of Origanum vulgare and Plectranthus amboinicus against anthracnose in coffee crops in southern Puerto Rico. Hypothesis: Origanum vulgare and Plectranthus amboinicus may be effective biological controls against fungi in coffee crops. Methods: Isolates of fungi were retrieved from sick coffee seeds. After purifying and identifying each isolate of fungi, we used bioinformatics analysis to develop the disc diffusion method. We established the fungal concentration as 0.5 using the standardized Mac Farland. Results: Isolates of Colletotrichum spp. were not present for evaluation; however, the antifungal activities of essential oregano oil (Origanum vulgare) were proven to inhibit isolates of Aspergillus flavus, Cladosporium spp., Fusarium solani and Aspergillus niger. Oregano brujo extract (Plectranthus amboinicus) did not produce halo of inhibition in any of the fungi being evaluated. Conclusion: Based on our results, oregano oil could be a viable solution for controlling coffee crop pathogens. andrea p. rivera torres centro de enseñanza e investigación en biotecnología y agrobiotecnología mentor: dra. gladys s. chompré gonzález antifungal activity of origanum spp essential oil and extract against anthracnose on coffee plants coffee antracnose origanum vulgare
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