Catálogo de Investigaciones | 2011-2012

CIENCIAS 44 Identification of Cytochrome p450 3a4 and 2c9 present in Cho Cells treated with a citrus hybrid Orangelo or best known as ‘Chironja’ is a hybrid citrus fruit between orange and grapefruit. Previous investigations agree that different compounds found in citrus fruits like orange and grapefruit inhibit, completely or partially, the activity of different isoenzymes in the cytochrome P450 system. These compounds are ascorbic acid, flavonoids, limonoids, furocoumarins and other related complexes. The effect of this hybrid in the P450 system of Chinese Hamster Ovarian Cells, especially in CYP3A4 and CYP2C9 isoenzymes was studied. Extraction from the fruit was performed by squeezing three chironjas. The skin of the fruit was analyzed by HPLC. CHO cells were treated for 48 hours with the chironja’s extract. After this procedure the luminescence of each sample was determined using an appropriate luminescent compound. Activity of the isoenzymes was measured. Detection and activity of CYP3A4 and CYP2C9 before and after the treatment was confirmed using HPLC technique. CYP3A4 and CYP2C9 activity and cytochrome P450 concentration was determined to establish the effect of the grapefruit extract on the isoenzymes. A decrease on enzyme activity was observed. Further studies are being conducted in order to establish the effect of this extract o the isoenzymes. natalia s. fernández dávila mentor dra. lizette santos santori cytochrome p450 isoenzymes orangelo cho cells