Catálogo de Investigaciones 2016-2017

catálogo de investigaciones | aÑo académico 2016-2017 45 Effects of Moringa oleifera formulas on 2D6 y 3A4 isoenzymes Moringa oleifera is a tree native to India, which is consumed as a nutritional supplement due to the fact that it provides minerals, vitamins and amino acids. It has been so popularized, that the pharmaceutical industry has commercialized it in capsules, although the consumers can also ingest it naturally as a tea. By taking these supplements without medical supervision, they can bring about not only positive but also negative effects in the way the enzymes are responsible for metabolizing the xenobiotic compounds work. Similarly, there may be some difference depending on the formulation used, whether leaf tea or commercial capsule. The cytochrome P450 system is responsible for metabolizing xenobiotic compounds. This system is composed of a superfamily of hemoproteins that catalyze a number of enzymatic reactions. The 2D6 and 3A4 isoenzymes have the function of metabolizing these compounds, such as M. oleifera . To detect the interaction of isoenzymes with M. oleifera , chinese hamster ovary cells were used. Once we treat the cells with the extracts of the capsule and the leaf at concentrations of 1.5, 2.5 and 3.5 mg / mL, the spectrophotometric tests were carried out to determine the activity of the isoenzymes. According to our results, the extracts of the capsule can be metabolized faster than the extracts of the leaf. Also, at concentrations higher than 2.5 mg / mL, in both extracts, the activity of the isoenzymes decreases. Therefore, M. oleifera affects the activity of 2D6 and 3A4 isoenzymes at concentrations greater than 2.5 mg / mL. The extract of the leaf is retained for a longer time in the system, which can lead to toxicity in the body. In future investigations, the activity of isoenzymes can be determined when they interact with extracts of roots or seeds. xiomara m. santiago narváez maestría en ciencias con especialidad en quiímica mentor: dra. lizette santos santori moringa oleifera isoenzyme 2d6 isoenzyme 3a4 cytochrome p450 hco cells