Catálogo de Investigaciones 2017-2018

CATÁLOGO DE INVESTIGACIONES | AÑO ACADÉMICO 2017-2018 45 Effects of cadmium-based quantum dots on cell viability and cytochrome P450 1A1, 2C9, and 3A4 Chinese hamster ovary cells In the last few years, quantum dots (QDs) of II-VI semiconductors have attracted a research interest in different fields of science and technology. QDs offer a lot of benefits but may pose risks to the environment and organisms. The exposition of organisms to QDs can result in negative effects in their organs, tissues, cells, and immune system due to their production of reactive oxygen species. In vitro and in vivo studies have demonstrated that the effects of nanoparticles in mammals can cause cytokine production, oxidative stress, apoptosis, and changes in gene expression. The focus of this study was to evaluate the effects of cadmium sulfide (CdS), cadmium sufide/selenide (CdSSe), and cadmium sulfide/ telluride (CdSTe) QDs on cell viability and cytochrome P450 (CYP) 1A1, 2C9, and 3A4 of Chinese hamster ovary (CHO) cells, by culturing CHO cells with cadmium-based QDs concentration. Citotoxicity was measured with the trypan blue exclusion assay and CellTiter-Glo® luminescent cell viability assay. Microscopy examination showed a strong green fluorescence of the CdSSe QDs indicating a possible incorporation of the QDs inside the cells. In addition, P450- GloTM assays showed a decrease of CYP1A1, 2C9, and 3A4 activity. micol borrero pabón maestría en ciencias con especialidad en química mentor: dr. luis álamo nole viability quantum dots cytochrome p450 cho cells