Catálogo de Investigaciones 2017-2018

52 CIENCIAS Study the effect of mitophagy- defective (atg32Δ) strains during Stationary Phase in Saccharomyces cerevisiae Autophagy involves the formation of autophagosomes in which delivery damage organelles or cytoplasmic contents to fuse with vacuoles for its degradation in yeast cells. Mitophagy is a type of autophagy, which eliminate dysfunctional mitochondrial. The mitochondria are organelles that carry out important metabolic process in the cell such as energy production, storage of calcium ions, and apoptosis. However, mitochondria are the main producer of reactive oxygen species (ROS) into the cell via oxidative phosphorylation (OXPHOS). Mitophagy is activated as a response to cellular stress such as nutrient starvation, oxidative stress, hypoxia or inflammation as survival mechanism. Studies have found a receptor protein, Atg32, localized in the outer membrane in mitochondria which confers specificity to the activation of mitophagy process in cells. In this project, the hypothesis is that the defects in mitophagy pathway affects the growth and viability in Saccharomyces cerevisiae . To asses this hypothesis, we used a wild type (WT) and atg32Δ strains of S.cerevisiae during stationary phase to determine cell concentration and viability using the hemocytometer and dye exclusion assay, respectively. The results did not show a significant difference in cell concentration and viability between WT and atg32Δ strain during stationary phase under rich carbon source and starvation conditions. These findings suggest that other compensatory mechanisms were operating to cell survival. kharina román rivera y eyshla correa martínez investigación especial en ciencias mentor: dra. ceidy torres ortiz mitophagy saccharomyces cerevisiae autophagy