CATÁLOGO DE INVESTIGACIONES | AÑO ACADÉMICO 2019-2020 115 Molecular Identification of Filamentous Fungi Diversity in North Coast Beaches Sands of Puerto Rico The objectives of this investigation were to assess the filamentous fungi diversity of four popular beaches; identify the genus and species; and identify the taxonomic relationship between the most abundant fungi. The beaches studied are located in the towns of Vega Baja, Manatí, Barceloneta and Arecibo. One sample of dry sand per month from three equidistant points was acquired every month for a year in each beach. The samples were homogenized according to dry (December-April) and humid (MayNovember) seasons, for a total of four composite samples per season. The DNA of each sample was isolated and quantified; and, upon sequencing, evaluated by metagenomics analysis with MG-RAST. The taxonomic analysis demonstrated that there is no relationship in the genus of the most abundant species. As significant finding, 66 species of new registries were identified, including Malassezia restricta, Arthrographics eremomycyces, and Cephaliophora tropica. Not only were many of the species pathogenic, several genera of filamentous fungi have been previously isolated from patients in nasal culture, and can cause eye, respiratory and skin disease. The majority of these fungi use direct contact and air transport as transmission vehicle to the host. Echevarría, L. (2019). Molecular identification of filamentous fungi diversity in North Coast beaches sands of Puerto Rico. PSM International Journal of Molecular Microbiology, 2(3), 51-61. https://journals.psmpublishers.org/index.php/ijmm/ article/view/313 dra. lourdes echevarría garcía departamento de biología y ciencias ambientales, pucpr psm international journal of molecular microbiology vol. 2 - issue 3, 2019 international conference on molecular biology and genetic engineering melbourne, australia pcr dna pathogen sand filamentous fungi
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