42 CIENCIAS In vitro progress of organogenesis of Citrus X Latifolia Citrus is vital in the food industry; its fruit is an essential source of vitamins, acids, and aromatic compounds. The demand for citrus cultures increased during the last year because of plant disease, and manufactured products. Farmers increased the culture of Citrus x Latifolia (Persian lime) because of its high demand due to the antioxidants content, ascorbic acid, citric acid, and flavonoid compounds. The demand for improved genetic material, and disease-free plants were the main problems that plant tissue culture techniques needed to solve. Biotechnology provided the necessary molecular tools to improve in vitro propagation during the last decade. Micropropagation is an in vitro technique producing identical clones yielding quality plants via organogenesis. Direct and indirect organogenesis used a medium with plant growth regulators, which could be utilized in a bioreactor by immersing multiple-node samples. Temporary immersion technology allowed high multiplication rate per explant and production of biological compounds. josé l. mercado rivera maestría en ciencias con especialidad en biotecnología mentor: prof.a rosa berrios villarini in vitro organogénesis citrus latifolia persian lime
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