#PIONEROSENINVESTIGACION building material and to protect the colony from parasites and pathogens. Despite its antimicrobial properties, recent studies showed that propolis hosts diverse microbial strains, some with great antimicrobial potential. In this study, the first description of the bacterial community of propolis produced by the gentle Africanized honeybee was reported. Propolis was sampled from hives of two different geographic areas of Puerto Rico (PR, USA), and the associated microbiota investigated by both cultivation and metataxonomic approaches. Metabarcoding analysis showed appreciable bacterial diversity in both areas and statistically significant dissimilarity in the taxa composition of the two areas, probably due to the different climatic conditions. Both metabarcoding and cultivation data revealed the presence of taxa already detected in other hive components and compatible with the bee’s foraging environment. Isolated bacteria and propolis extracts showed antimicrobial activity against Gram-positive and Gram-negative bacterial tester strains. These results support the hypothesis that the propolis microbiota could contribute to propolis’ antimicrobial properties. Revista: Microorganisms Año de publicación 2023 Número 11(5), pág. 1130 Página web: https://www.mdpi.com/journal/ microorganisms DOI: https://doi.org/10.3390/ microorganisms11051130 País de la revista: Suiza Versión pdf: https://www.mdpi.com/20762607/11/5/1130/pdf Dra. Yaliz Loperena Álvarez Título: Cryptococcus gattii Can Use the Cacti Pilosocereus sp. to Grow, Develop a Capsule and Reduce Melanin in Vitro Investigadores: Paola Ramos-Irizarry- John Hopkins University Bárbara Sánchez- University of Puerto Rico, Mayagüez campus Yaliz Loperena-Álvarez- Pontifical Catholic University of Puerto RicoMayagüez campus Resumen: Cryptococcus gattii is a pathogenic yeast, member of the C. neoformans/ gattii complex.Previous work from our laboratory has established the presence of C. gattii on cacti lesions, providing proof that it can grow in a stressful environment. However, it is not known which part of the cactus the yeast uses for nutrients. The purpose of this research is to determine the ability of C. gattii to grow in different parts of the cactus to assess how the yeast adapts to grow in this unique environment. Cactus media were developed using the outer, inner, and whole cactus from Pilosocereus spp. Cryptcoccus gattii was grown on the different cactus media, along with potato dextrose agar as a control for 24 and 48 h at 30 C. Compared to the control medium, yeast growth was reduced in all cactus media, while an increase in the capsule development of the yeast grown in the inner part and the whole-cactus media was observed. Interestingly, the yeast produces melanin when grown in the outer membrane medium, which is dependent on laccase, suggesting that the outer membrane may contain a precursor that stimulates pigment production. To our knowledge, this is the first study addressing these key differences in the growth of C. gattii on different parts of the cactus. Revista: Microbiology Research Volumen- 14 DOI–10.3390/microbiolres14020056 País de la revista– Basel, Switzerland. viene de la página anterior 36 HUELLAS DEL FUTURO INFORMA
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