Primavera otoño 2020 (Año LXIII Núms. 122-123) Año LXIV Núm. 124-125 horizontes PRIMAVERA / OTOÑO 2021 PUCPR 63 differential influence of rootstocks on CLas- tolerance of the trees with sensitive scion (upper part of a plant) such as the sweet orange, the increased generalized health of the rootstock is not associated with the CLas infection. Zambon et al. (2019) described overdose treatments as a therapeutic for citrus. They over-dosed with boron and manganese, determining lower potassium content in the soil during September. Potassium indicated an ionic radius greater than boron and manganese. Root propensity was significant, which caused loss of potassium in the roots. Manganese treatment obtained higher contents during the same month, suggesting negative interactions between boron and manganese. Thus, HLB-infested trees benefited from overdoses of micronutrient supplements. The results provide a tool to accelerate the evaluation of the new rootstock and other HLB mitigation strategies during the preliminary stages by replacing them in long-term trials with 50-week greenhouse testing. CLas was identified as having five copies of nrdB, three in designated form of nrdBs and nrdA. Zheng et al. (2016) speculated that the non-functional short form of nrdBS generated a heterodimer as part of RNR regulation in proliferation. According to the information obtained, the β-subunit of ribonucleotide reductase provided information on the biology of CLas. Researchers demonstrated the absolute quantification and amplification of a single pathogen using two multi-copy genes. Otherwise, they encouraged researchers studying model proteobacteria to participate in combatting the agricultural plague. Conclusion After reading and interpreting this manuscript, we can observe that various vectors affect citrus plants worldwide. Scientists will continue finding ways to inhibit Candidatus Liberibacter asiaticus without damaging the fruit or the tree. We as human beings can continue to care for infected trees without having to cut them down. Target Gene Primer/ Probe name Amplification 16S rRNA gene 16S F 16S rRNA gene nrdB, B- subunit of ribonucleotide reductase RNR F nrdB, B- subunit of ribonucleotide reductase Figure 1: Primer and probe sequence used for qPCR and ddPCR assay for the dection of “ Candidatus Liberibacter asiaticus.” Acknowledgments I would like to acknowledge the original concept by Ana E. Perez Matos and editing of this manuscript by Dr. Dallas E. Alston, professor in the Department of Natural Sciences, PUCPR. References Attaran, E., Berim, A., Killiny, N., Beyenal, H., Gang, D. R., & Omsland, A. (2020). Controlled replication of ‘Candidatus Liberibacter asiaticus’ DNA in citrus leaf discs. Microbial Biotechnology, 6(3), 747- 759. https://sfamjournals.onlinelibrary . 7915.13531 Bao, M., Zheng, Z. , Sun, X. , Chen, J. , & Deng X. (2020). Enhancing PCR capacity to detect ‘Candidatus Liberibacter asiaticus’ utilizing whole genome sequence information. The American Phytopathological Society, 104, 527-532. 10.1094/PDIS-05-19-0931-RE